About the Author(s)


Unarine B. Makungo Email symbol
Division of Public Health Surveillance and Response, National Institute for Communicable Diseases, Johannesburg, South Africa

Public Health Directorate, Limpopo Department of Health, Polokwane, South Africa

Tshilidzi E. Ramutshila symbol
South African Field Epidemiology Training Program, National Institute for Communicable Diseases, Johannesburg, South Africa

School of Health Systems and Public Health, University of Pretoria, South Africa

Mantwa C. Mabotja symbol
Division of Public Health Surveillance and Response, National Institute for Communicable Diseases, Johannesburg, South Africa

School of Public Health, University of Witwatersrand, Johannesburg, South Africa

Juno Thomas symbol
Centre for Enteric Diseases, National Institute for Communicable Diseases, Johannesburg, South Africa

Ruth Lekalakala-Mokaba symbol
Department of Medical Microbiology, National Health Laboratory Services, Polokwane, Limpopo, South Africa

Department of Medical Microbiology, University of Limpopo, Polokwane, South Africa

Anthony M. Smith symbol
Centre for Enteric Diseases, National Institute for Communicable Diseases, Johannesburg, South Africa

Joy Ebonwu symbol
Division of Public Health Surveillance and Response, National Institute for Communicable Diseases, Johannesburg, South Africa

Shannon L. Williams symbol
Centre for Enteric Diseases, National Institute for Communicable Diseases, Johannesburg, South Africa

Jimmy Khoza symbol
Division of Public Health Surveillance and Response, National Institute for Communicable Diseases, Johannesburg, South Africa

Queen Ranoto symbol
Public Health Directorate, Limpopo Department of Health, Polokwane, South Africa

Ntshengedzeni Muvhango symbol
Department of Public Health Medicine, Limpopo Department of Health, Polokwane, South Africa

Mmatjatji Mosoma symbol
Sekhukhune District, Limpopo Department of Health, Polokwane, South Africa

Elizabeth Phokane symbol
Sekhukhune District, Limpopo Department of Health, Polokwane, South Africa

Genevie Ntshoe symbol
Division of Public Health Surveillance and Response, National Institute for Communicable Diseases, Johannesburg, South Africa

Katherine Calver symbol
Division of Public Health Surveillance and Response, National Institute for Communicable Diseases, Johannesburg, South Africa

Vivien Essel symbol
Division of Public Health Surveillance and Response, National Institute for Communicable Diseases, Johannesburg, South Africa

Marlene F. Ngobeni symbol
Public Health Directorate, Limpopo Department of Health, Polokwane, South Africa

Kerrigan McCarthy symbol
Division of Public Health Surveillance and Response, National Institute for Communicable Diseases, Johannesburg, South Africa

Citation


Makungo UB, Ramutshila TE, Mabotja MC, et al. Epidemiological investigation of a typhoid fever outbreak in Sekhukhune District, Limpopo province, South Africa – 2017. S Afr J Infect Dis. 2020;35(1), a107. https://doi.org/10.4102/sajid.v35i1.107

Research Project Registration:

Project Number: LPREC/19/2018: PG

Original Research

Epidemiological investigation of a typhoid fever outbreak in Sekhukhune District, Limpopo province, South Africa – 2017

Unarine B. Makungo, Tshilidzi E. Ramutshila, Mantwa C. Mabotja, Juno Thomas, Ruth Lekalakala-Mokaba, Anthony M. Smith, Joy Ebonwu, Shannon L. Williams, Jimmy Khoza, Queen Ranoto, Ntshengedzeni Muvhango, Mmatjatji Mosoma, Elizabeth Phokane, Genevie Ntshoe, Katherine Calver, Vivien Essel, Marlene F. Ngobeni, Kerrigan McCarthy

Received: 28 May 2019; Accepted: 07 June 2020; Published: 16 Nov. 2020

Copyright: © 2020. The Author(s). Licensee: AOSIS.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background: Typhoid fever remains a public health concern in South Africa, where the risk of transmission is high because of poor access to safe water and sanitation. This study describes the investigation of typhoid fever outbreak in Limpopo province.

Methodology: Following notification of laboratory-confirmed cases, a descriptive study was conducted at Sekhukhune District, Limpopo province. A suspected case was defined as any person residing in Makhuduthamaga Municipality from November 2017 to January 2018, presenting with fever and gastrointestinal symptoms. Data were collected using case investigation forms. Whole-genome sequencing (WGS) was carried out on Salmonella Typhi isolates and polymerase chain reaction (PCR) test was done for Salmonella species from water samples. Location of cases and water sources were mapped using ArcGIS mapping tool.

Results: Amongst 122 cases, 54% (n = 66) were female and 6% (n = 7) laboratory-confirmed. The median age of the cases was 11 years (range 2–83 years), with 79% (n = 102) being children under the age of 14 years. Salmonella species were detected in 37% (10/27) of water samples and geographic information system (GIS) mapping showed clustering of cases in Tswaing-Kgwaripe and Vlakplaas villages. Six isolates were available for WGS analysis, with resulting data showing that five of the six isolates were genetically related. Phylogenetic analysis showed that the five isolates clustered together were genetically related showing < 22 single nucleotide polymorphisms when compared to each other.

Conclusion: Molecular epidemiology of isolates suggests a common source outbreak, supported by the detection of Salmonella species from water sources. Consumption of water from contaminated open water sources, because of ongoing interruption of municipal water supply, was the likely cause of the outbreak. The investigation highlights the importance of consistent safe water supply and the ability of district surveillance systems to identify and contain outbreaks.

Keywords: typhoid fever; open water sources; outbreak; Limpopo; South Africa.

Introduction

Water scarcity is ranked as the foremost crisis locally and globally.1 South Africa has made a certain degree of improvement on the establishment of sanitation and water quality infrastructures, which has played a fundamental role in the significant reduction of pathogens responsible for water and foodborne illnesses. Despite these improvements, most rural and peri-urban areas experience recurrent water and foodborne disease outbreaks, which have a negative impact on human health.2,3 The ongoing state of climate change accompanied by drought has become a fuel for majority of typhoid fever outbreaks in areas with poor sanitation and lack of safe water.4,5,6

Typhoid fever is an epidemic-prone disease caused by a gram-negative bacterium, Salmonella Typhi and Salmonella Paratyphi. It is transmitted through consumption of food or water contaminated by faeces or urine of infected persons. Typhoid fever often presents with gastrointestinal symptoms (diarrhoea, abdominal cramps) and life-threatening, enteric febrile systemic illnesses necessitating prompt antibiotic treatment to prevent complications.7

Typhoid fever is a life-threatening disease. Accurate diagnosis in the early stage and effective surveillance are important to ensure early detection and appropriate treatment to mitigate the spread of disease.7 A definitive diagnosis of typhoid fever requires the isolation of Salmonella Typhi from blood, bone marrow or a specific anatomical lesion. Blood culture is a recommended diagnostic test of choice given the limitations of testing other specimen types.8 Low- and middle-income countries with high typhoid fever endemicity are still using Widal test because it is relatively cheaper and easy to perform. However, the Widal test is not a recommended confirmatory test because of its low sensitivity, specificity and positive predictive value.9,10

The World Health Organization (WHO) estimates that approximately 21 million cases and 222 000 typhoid-related deaths occur annually worldwide.11 South Africa has observed mixed patterns of endemic disease and sporadic cases in more industrialised areas of the country through active laboratory-based surveillance, conducted by GERMS-SA of the National Institute for Communicable Diseases (NICD).12 The two largest typhoid fever outbreaks in the country occurred in Delmas, Mpumalanga province in 1993 and 2005, where over 1000 and 600 cases were reported, respectively.13 However, the number of cases and deaths has declined over the last 22 years, from 6000 cases in 1985 to 200 cases in 2002.12,14

On 15 November 2017, Sekhukhune District Department of Health was notified of a laboratory-confirmed Salmonella Typhi case from a tertiary hospital in the province. There were reports of over 20 suspected cases in one of the district hospitals in Sekhukhune District. This led to the activation of the district outbreak response team from 21 November to 31 December 2017. A descriptive epidemiological investigation was conducted to verify the existence of an outbreak, identify the source infection and implement recommendations for the control and prevention of typhoid fever spread in Sekhukhune District.

Methods

Study design and population

A cross-sectional descriptive study was conducted to investigate a typhoid fever outbreak amongst 122 patients that presented with clinically compatible illness for typhoid fever at local health facilities in Makhuduthamaga Local Municipality at Sekhukhune District, Limpopo province, from mid-November 2017. Sekhukhune District is divided into five subdistricts (i.e. Greater Tubatse, Makhuduthamaga, Ephraim Mogale, Elias Motsoaledi and Fetakgomo). The geographical land distribution is 2096.60 km2, with a population of 274 358 according to the 2011 local municipality census (Figure 1).15

FIGURE 1: Sekhukhune map.15

The following case definitions were used to identify cases. A suspected case was defined as any person who resided in Makhuduthamaga Local Municipality and presented with measured fever of +38°C with acute onset of fever and gastrointestinal symptoms during November and December 2017. A confirmed case was defined as a suspected case who had isolation of typhoidal Salmonella serovars from stool or blood specimens.16

Data collection and tools
Epidemiological investigations

An epidemiological investigation was conducted by reviewing clinical records and through a standardised case investigation form (CIF). Collected data included demographics, clinical presentation, laboratory results, travel history, food preparation practices, consumption of restaurant foods, sanitation practices and source of drinking water. Data on the location of the case-patients and the sources of water were collected in January 2018 using the GIS map, focusing on the homes of case-patients, surrounding schools, crèches and sources of water.

Clinical and laboratory investigations

The clinicians working at the health facilities, where suspected cases presented, collected clinical samples to rule out typhoid fever. National Health Laboratory Services (NHLS) received 106 clinical specimens from 15 November to 31 December 2017. The NHLS microbiology laboratory at Pietersburg Provincial Hospital received blood (n = 65), stool (n = 6) and rectal swabs (n = 3) for culture. In addition, blood samples were collected and Widal tests (n = 32) were requested in instances where blood culture bottles were unavailable.

Six laboratory-confirmed isolates were submitted to the NICD’s Centre for Enteric Diseases (CED) for confirmation of a diagnosis of Salmonella Typhi and whole-genome sequencing (WGS) analysis. The CED received bacteria on Dorset-egg transport media [Diagnostic Media Products (DMP), NHLS, Johannesburg, South Africa], and then subcultured onto 5% blood agar (DMP), to check for viability and purity. Cultures were identified using standard phenotypic microbiological identification and serotyping techniques, which are briefly described here. As required, bacterial colonies were identified using the VITEK-2 COMPACT 15 automated microbial identification system (bioMérieux, Marcy-l’Étoile, France). Serotyping was performed according to the White–Kauffmann–Le Minor scheme. Antimicrobial susceptibility testing was performed using the VITEK-2 COMPACT 15 system (bioMérieux) and the Etest method (bioMérieux).

For WGS analysis of bacteria, the methodology is briefly outlined here. Genomic DNA was isolated from bacteria using the Qiagen QIAamp DNA Mini Kit (Qiagen, Hilden, Germany). DNA libraries were prepared using a Nextera XT DNA Library Preparation Kit (Illumina, San Diego, CA, USA), followed by a 2 × 300 paired-end sequencing runs with 100× coverage using Illumina MiSeq equipment. Raw data generated on the MiSeq were further analysed using tools available in the CLC Genomics Workbench Software, version 8.5 (Qiagen). Using the ‘Trim Sequences Tool’, sequence reads were trimmed to include quality trimming, ambiguity trimming and length trimming to discard reads below a length of 50 bases. Trimmed reads were assembled using the ‘De novo Assembly Tool’; the assembly algorithm works by using de Bruijn graphs to produce contiguous (contig) sequences (minimum contig length was set at 200 bases). For phylogenetic analysis of bacteria, assembled genome data were analysed using the ‘CSI Phylogeny 1.4’ on-line analysis pipeline available at the Center for Genomic Epidemiology (CGE) of the Technical University of Denmark (http://www.genomicepidemiology.org/). The CSI Phylogeny pipeline uses various publicly available programs and the analysis steps are briefly described as follows: assembled genome data is aligned against a reference genome and single nucleotide polymorphisms (SNPs) are called; SNPs are filtered and qualified; final qualified SNPs for each genome are concatenated to an alignment; and phylogeny is then inferred based on a comparison of SNP alignments of strains. Single nucleotide polymorphisms were called by alignment and referencing against a South African strain isolated in 2016 (reference number TCD981492). Single nucleotide polymorphism alignments were analysed with iTOL software to generate phylogenetic maximum-likelihood trees.

Environmental investigations

Water sampling sites were guided by information from case-patients’ interviews. Water samples were collected by environmental health practitioners (EHPs) and tested for Salmonella species using polymerase chain reaction (PCR) method at the Council for Scientific and Industrial Research (CSIR) laboratory.

Geographic information system mapping

Geographic information system (GIS) mapping of the case-patients’ locations and water sources was conducted by a trained NICD team and Sekhukhune Department of Health district staff (health promotion coordinator and ward-based outreach team), which was led by a GIS specialist, at all affected villages. Demographic and Global Positioning System (GPS) coordinates were collected and color-coded points were used to indicate cases (confirmed, suspected) and water sources tested for Salmonella species.

Health promotion activities

Health Promotion activities were conducted during the outbreak through door-to-door consultations, community gatherings at the 13 affected villages, local radio station and primary health facilities. Ward committee councillors were engaged by Sekhukhune outbreak response team to facilitate awareness on food and waterborne illness.

Clinical in-service training activities

Typhoid fever clinical management training was conducted by District Health Special Program Unit and NICD staff during and after the outbreak. The training was conducted because of observed non-uniformity and challenges on diagnosis and treatment of typhoid fever cases by clinicians.

Data analysis

Categorical data were summarised as proportions. Descriptive statistics (median and range) were used to describe continuous variables. The epidemic curve was created using the date of onset of symptoms (Figure 2).31 Data management and analysis were performed using Microsoft Excel 2016.

FIGURE 2: Presenting signs and symptoms amongst all cases, Sekhukhune District, Limpopo province, November – December 2017.

Ethical consideration

The study was approved by the Limpopo Provincial Research Ethics Committee Organization: Office of the Premier (Clearance number: LPREC/19/2018: PG).

Results

Epidemiological findings

Between 15 November and 31 December 2017, a total of 122 cases were reported, of which 6% (7/122) were laboratory-confirmed for Salmonella Typhi by blood culture. The patient characteristics are described in Table 1. The median age of the cases was 11 years (range 2–83 years), with most cases between 5 and 14 years (64%, 78/122). Females accounted for 54% (66/122) of the cases. Majority of cases were from Tswaing village (67%, 82/122), with four laboratory-confirmed cases. Most case-patients (67%, 82/122) reported obtaining water for domestic use from irrigation furrows and other untreated water sources because of interrupted municipal water supply (Table 1).31 The common presenting symptoms were fever (47%, 90/122), diarrhoea (43%, 52/122) and abdominal pain (34%, 41/122). The epidemic curve of all the cases by date of onset is presented in Figure 3.31 The epidemic curve suggests the outbreak started in early November and peaked between the second and third weeks.

FIGURE 3: Epidemic curve of typhoid fever cases (suspected and confirmed) by date of onset, Sekhukhune District, Limpopo province, November – December 2017 (N = 122).

TABLE 1: Characteristics of typhoid fever cases, Sekhukhune District, Limpopo province, November – December 2017 (N = 122).
Laboratory analysis of isolates

Six isolates were available for WGS analysis, with resulting data showing that five of the six isolates were genetically related. Phylogenetic analysis showed that the five related isolates clustered together, with isolates showing <22 SNPs when compared to each other. Of the six isolates sent for WGS, five isolates that were obtained from cases in Tswaing and Strydkraal villages were genetically related (<22 SNPs), as shown in Figure 4.31 A single, un-clustered strain was closely related to the 2016 Zimbabwe typhoid fever outbreak. All six isolates were susceptible to ciprofloxacin and ceftriaxone.

FIGURE 4: Snapshot from a maximum likelihood phylogenetic tree drawn using single nucleotide polymorphism alignments from whole-genome sequencing data of Salmonella Typhi isolates from South Africa. The blocked region highlights a cluster of related isolates sourced at the Sekhukhune District, Limpopo province, November to January 2018.

Environmental findings

Of the seven confirmed cases, 86% (n = 6) reported using water from the open water sources. Pathogenic Salmonella species were detected in 10/27 (37%) samples from water sources in the district. The GIS mapping showed clustering of cases in Village S, with 4/7 (57%) laboratory-confirmed cases and 78/122 (64%) suspected cases (Figure 5).

FIGURE 5: Geographic distribution of suspected and confirmed typhoid fever cases and water sources, Sekhukhune District, Limpopo province, November – December 2017.

Discussion

This outbreak investigation discovered that rural communities are still at a disadvantage for basic human rights such as safe water and sanitation despite national infrastructure plans in place. Consumption of contaminated water from untreated open sources was the likely driver of infection in this outbreak. This was evidenced by six of the seven laboratory-confirmed cases where water was reported to have been used from open sources for drinking and other household activities. A relation between typhoid fever and contaminated water sources has been identified in other studies.17,18 Unsafe disposal of excreta and solid waste might have led to the contamination of open water sources, which has been reported in other settings.19,20 The geographical location of water sources sampled in our investigation is in close proximity to villages where cases are clustered. The use of open source drinking water by residents of affected villages is the most likely factor to have resulted in the typhoid fever outbreak. These findings are consistent with results of previous similar outbreak investigations.13,19,21

The sudden increase in cases after the start of the investigation was likely because of active community outreach and awareness about typhoid fever signs, symptoms and prevention. A steady decline in cases from the last week of November was probably because of heightened community awareness, high suspicion index at treating hospitals and fixing of the broken down water plant by the local municipality, as well as provision of free water purification products and clean water. This is indicative of how a multisectoral approach impacts mitigation of disease outbreaks.5,22 The investigation highlights the importance of consistent safe water supply, and the ability of district surveillance systems to identify and contain outbreaks.

A high burden of typhoid fever is often observed mostly in the paediatric population in low- and middle-income countries.23,24 In our study, 75% (92/122) of the cases were under 15 years of age. Although no deaths were reported during this outbreak, a high burden of typhoid fever in children is a public health concern, as there is a > 4 time’s risk of mortality because of increased risk of complications and prolonged hospitalisation that has been observed in other settings.25,26 The high burden of typhoid fever amongst children in this study specifies the high-risk population but also enables policymakers and municipal service delivery sectors to make informed decisions on prioritisation of safe water supply in schools and the community in general.

Knowledge about typhoid fever clinical cases management and public health response is fundamental to how well typhoid outbreaks are contained and prevented. Definitive diagnosis of typhoid fever, such as isolation of Salmonella Typhi from blood culture, is largely available and affordable in South Africa.16 The use of Widal test may result in misdiagnosis and mismanagement of patients.9,10,27 During the investigation period, in-service training on clinical management and public health response of typhoid fever were conducted in the district after it became known that there was no uniformity in patient management amongst clinicians. Typhoid fever management guidelines were distributed to all hospitals to ensure the proper management of patients.16,28

Recommendations

The following recommendations were given to the provincial and local authorities.

  • Department of Health, Department of Water Affairs and municipality should form an intersectoral committee that facilitates communication between departments and ensures that challenges pertaining to access to clean water and sanitation are addressed promptly.
  • Purification and chlorination of public water supplies should be monitored by the intersectoral health, water and sanitation committee.
  • There should be continuous community health education and awareness on water, sanitation and hygiene practices.
  • Case management should include contact tracing with an investigation for asymptomatic carriage, documentation of eradication of carriage, and restrictions on food handling practices amongst laboratory-confirmed cases until at least three consecutive negative stool cultures have been taken.
  • Health care workers should complete standardised CIFs to ensure that high quality data is collected.
  • Department of Health should develop a case control outbreak investigation protocol to guide field workers and to ensure more robust future investigations.

Limitations

The study had a few limitations. The PCR-based test used by CSIR to test environmental water samples detects all Salmonella enterica subspecies as well as Salmonella bongori, but it cannot distinguish between typhoidal and non-typhoidal Salmonella species.29,30

Because of the nature of the cross-sectional study design, we could not determine risk factors that contributed to typhoid fever infection.

Conclusion

This descriptive study highlights the importance of outbreak investigation and management of typhoid fever cases to ensure disease control. The observed decline of cases further highlights that access to clean water, a multisectoral approach and communication amongst different stakeholders in conjunction with training of health professionals on diagnosis and management of waterborne diseases have an impact on the management of epidemic-prone diseases.

Acknowledgements

All parties mentioned below were involved in the outbreak investigation and response in terms of their departmental expertise:

  • Limpopo Department of Health
  • Makhuduthamaga Local Municipality
  • Sekhukhune District Municipality
  • Sekhukhune District: Department of Water Affairs
  • Sekhukhune District: Outbreak response teams
  • Limpopo province: Outbreak response team
  • Makhuduthamaga mobile clinic teams
  • Makhuduthamaga subdistrict: Community
  • National Health Laboratory Services
  • National Institute for Communicable Diseases.
Competing interests

The authors have declared that no competing interests exist.

Authors’ contributions

All authors contributed equally to this work.

Funding information

This research received no specific grant from any funding agency in the public, commercial or not-for-profit sectors.

Data availability statement

Anonymised data for this work is available on request.

Disclaimer

The views and opinions expressed in this article are those of the authors and do not necessarily reflect the official policy or position of any affiliated agency of the authors.

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