Original Research

Evaluation of the Copan eSwab®, a liquid-based microbiology transport system, for the preservation of Neisseria gonorrhoeae at different temperatures

Lindy Gumede, Frans Radebe, Duduzile Nhlapo, Venessa Maseko, Tendesayi Kufa-Chakezha
Southern African Journal of Infectious Diseases | Vol 32, No 3 | a46 | DOI: https://doi.org/10.4102/sajid.v32i3.46 | © 2019 Lindy Gumede, Frans Radebe, Duduzile Nhlapo, Venessa Maseko, Tendesayi Kufa-Chakezha | This work is licensed under CC Attribution 4.0
Submitted: 14 May 2019 | Published: 01 October 2017

About the author(s)

Lindy Gumede, National Institute for Communicable Diseases, Johannesburg, South Africa
Frans Radebe, National Institute for Communicable Diseases, Johannesburg, South Africa
Duduzile Nhlapo, National Institute for Communicable Diseases, Johannesburg, South Africa
Venessa Maseko, National Institute for Communicable Diseases, Johannesburg, South Africa
Tendesayi Kufa-Chakezha, National Institute for Communicable Diseases, Johannesburg, South Africa

Full Text:

PDF (140KB)

Share this article

Bookmark and Share

Abstract

Aims and objectives: To evaluate the survival duration of Neisseria gonorrhoeae from male urethral discharge specimens collected using the Copan eSwab® liquid-based microbiology transport system, at both ambient and refrigerator temperatures.

Methods: Three urethral swabs (one Dacron, two Copan eSwabs®) were collected from each male patient presenting with purulent urethral discharge to a community-based primary healthcare centre in Johannesburg. The Dacron swab was directly inoculated onto New York city agar medium, and the Copan eSwabs® transported and held at room and refrigerator temperature, for daily sub-culture onto New York city agar over a total period of seven days (168 h). The utility of Copan eSwabs® for the transport and survival of N. gonorrhoeae at different temperatures was determined by comparison to culture obtained by ‘gold standard’ direct plate inoculation.

Results: N. gonorrhoeae isolation rates from Copan eSwabs® at fridge temperature and ambient temperature were as follows: 87.9% vs 79.3% at 48 h; 67.2% vs 60.3% at 72 h; 60.3% vs 22.4% at 96 h; and, 53.4% vs 3.4% at 120 h, respectively. The viability of subculture decreased significantly from eSwabs® maintained at room temperature from 96 h onwards of specimen collection.

Conclusion: To ensure the preservation and an acceptable isolation rate of N. gonorrhoeae from urethral discharge specimens, Copan eSwabs® should be transported and maintained at refrigerator temperatures, and must reach the processing laboratory by at least 120 h (5 days) after collection.


Keywords

amies transport medium; Copan eSwab; Neisseria gonorrhoeae

Metrics

Total abstract views: 410
Total article views: 138


Crossref Citations

No related citations found.