Original Research

The recovery and molecular identification of HAdV-D17 in raw sewage and mussel samples collected in the Eastern Cape province of South Africa

Hillary J. Vos, Caroline M. Knox
Southern African Journal of Infectious Diseases | Vol 33, No 1 | a27 | DOI: https://doi.org/10.4102/sajid.v33i1.27 | © 2019 Hillary J. Vos, Caroline M. Knox | This work is licensed under CC Attribution 4.0
Submitted: 13 May 2019 | Published: 31 March 2018

About the author(s)

Hillary J. Vos, Department of Biochemistry and Microbiology, Rhodes University, Grahamstown, South Africa
Caroline M. Knox, Department of Biochemistry and Microbiology, Rhodes University, Grahamstown, South Africa

Full Text:

PDF (178KB)

Abstract

Human adenoviruses (HAdV) are a common cause of clinical infections in South Africa. However, there is a lack of information regarding the prevalence and molecular identification of this virus in the environment. The objective of this study was to investigate the recovery and molecular identification of HAdV in sewage and mussel samples. All samples were subjected to transmission electron microscopy, viral DNA extraction and nested PCR amplification using adenovirus-specific primers targeting a conserved region of the hexon gene. Amplicons were cloned and sequenced and BLAST analysis revealed a closest matched sequence (98% identity) belonging to HAdV-D17.

Keywords

Human adenovirus (HAdV); mussels; nested PCR; raw sewage

Metrics

Total abstract views: 3221
Total article views: 1422

 

Crossref Citations

1. The First Detection of Human Bocavirus Species 2 and 3 in Raw Sewage and Mussels in South Africa
Oikwathaile Onosi, Nicole S. Upfold, Michael D. Jukes, Garry A. Luke, Caroline Knox
Food and Environmental Virology  vol: 12  issue: 1  first page: 84  year: 2020  
doi: 10.1007/s12560-019-09417-w

2. Occurrence of Human Enteric Viruses in Water Sources and Shellfish: A Focus on Africa
Nicole S. Upfold, Garry A. Luke, Caroline Knox
Food and Environmental Virology  vol: 13  issue: 1  first page: 1  year: 2021  
doi: 10.1007/s12560-020-09456-8

3. Prevalence of Enteric Viruses in Raw Seafood Collected from the Retail Shops in Tehran, Iran, 2022–2023
Golshid Javdani Shahedin, Maryam Yazdanizad, Seyed Davood Mousavi Nasab, Delaram Doroud, Rahman Shokri, Ramin Mazaheri Nezhad Fard
Iranian Journal of Medical Microbiology  vol: 18  issue: 3  first page: 190  year: 2024  
doi: 10.30699/ijmm.18.3.190