Original Research

The recovery and molecular identification of HAdV-D17 in raw sewage and mussel samples collected in the Eastern Cape province of South Africa

Hillary J. Vos, Caroline M. Knox
Southern African Journal of Infectious Diseases | Vol 33, No 1 | a27 | DOI: https://doi.org/10.4102/sajid.v33i1.27 | © 2019 Hillary J. Vos, Caroline M. Knox | This work is licensed under CC Attribution 4.0
Submitted: 13 May 2019 | Published: 31 March 2018

About the author(s)

Hillary J. Vos, Department of Biochemistry and Microbiology, Rhodes University, Grahamstown, South Africa
Caroline M. Knox, Department of Biochemistry and Microbiology, Rhodes University, Grahamstown, South Africa

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Abstract

Human adenoviruses (HAdV) are a common cause of clinical infections in South Africa. However, there is a lack of information regarding the prevalence and molecular identification of this virus in the environment. The objective of this study was to investigate the recovery and molecular identification of HAdV in sewage and mussel samples. All samples were subjected to transmission electron microscopy, viral DNA extraction and nested PCR amplification using adenovirus-specific primers targeting a conserved region of the hexon gene. Amplicons were cloned and sequenced and BLAST analysis revealed a closest matched sequence (98% identity) belonging to HAdV-D17.

Keywords

Human adenovirus (HAdV); mussels; nested PCR; raw sewage

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