Short Article

Molecular detection and characterisation of mumps virus in cerebrospinal fluid in a Gauteng laboratory

Marieke Brauer, Marianne Wolfaardt, Lynne M. Webber, Maureen B. Taylor
Southern African Journal of Infectious Diseases | Vol 31, No 1 | a102 | | © 2019 Marieke Brauer, Marianne Wolfaardt, Lynne M. Webber, Maureen B. Taylor | This work is licensed under CC Attribution 4.0
Submitted: 16 May 2019 | Published: 31 March 2016

About the author(s)

Marieke Brauer, Faculty of Health Sciences, Department of Medical Virology, University of Pretoria, Pretoria, South Africa; National Health Laboratory Service, Tshwane Academic Division, Pretoria, South Africa
Marianne Wolfaardt, Faculty of Health Sciences, Department of Medical Virology, University of Pretoria, Pretoria, South Africa
Lynne M. Webber, Faculty of Health Sciences, Department of Medical Virology, University of Pretoria, Pretoria, South Africa; National Health Laboratory Service, Tshwane Academic Division, Pretoria, South Africa
Maureen B. Taylor, Faculty of Health Sciences, Department of Medical Virology, University of Pretoria, Pretoria, South Africa

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Abstract

The study aimed to determine the presence of mumps virus (MuV) in cerebrospinal fluid (CSF) specimens and to genetically characterise detected MuV strains. A real-time reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the MuV F gene, and characterisation was performed by sequencing of the SH gene. Mumps virus was detected in 1.2% (3/260) of specimens. Phylogenetic analysis of one MuV strain revealed that it clustered with the Jeryl-Lynn and RIT4385 vaccine strains. As far as the authors could ascertain this is the first study to provide viral proof that these vaccine-like strains may be associated with aseptic meningitis.

Keywords

aseptic meningitis; central nervous system; cerebrospinal fluid; genotype; polymerase chain reaction

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